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Nucleases

Ribonucleases In Rna Maturation And Degradation

The expression of genes into protein products requires the generation of a messenger RNA (mRNA) by transcription and the subsequent translation of the mRNA into protein. In bacteria, the mRNA is transcribed, translated, and then degraded by ribonucleases in rapid succession. Thus, the ribonucleases are primarily responsible for mRNA degradation in bacteria. In animal cells, RNA molecules are transcribed as precursors that require processing by ribonucleases to generate functional RNAs. This RNA maturation process requires cleavage by endonucleases and trimming by exonucleases. After the mRNA is translated into protein it is degraded by additional ribonucleases.

Fred Perrino

Bibliography

Gerlt, John A. "Mechanistic Principles of Enzyme-Catalyzed Cleavage of Phosphodiester Bonds." In Nucleases, 2nd ed., Stuart M. Linn, R. Stephen Lloyd, and Richard J. Roberts, eds. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press, 1993.

Inhalation of recombinant human DNA endonuclease breaks up sticky airway secretions in cystic fibrosis.

Additional topics

Medicine EncyclopediaGenetics in Medicine - Part 3Nucleases - The Nuclease Mechanism, Deoxyribonucleases In Dna Replication And Repair, Ribonucleases In Rna Maturation And Degradation