Transposable Genetic Elements
Transposons As Molecular Biology Tools
Transposons can be used to facilitate cloning of genes, identify regulatory elements, and produce transgenic organisms. For example, transposon tagging involves inducing transposition of a TE, allowing for disruption of a gene that generates an organism with a mutant phenotype, and is followed by molecular techniques that allow for the identification of the gene. A variation of transposon tagging (enhancer trapping) uses P elements to identify DNA sequences that regulate the expression of genes. P elements can also be used to incorporate foreign genes into fruit flies (transgenics). In addition, transposon fossils have been useful for the isolation of species-specific DNA from complex sources such as using inter-Alu PCR for the isolation of human genomic DNA sequences.
David H. Kass
and Mark A. Batzer
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Medicine EncyclopediaGenetics in Medicine - Part 4Transposable Genetic Elements - Early Evidence, Tes Across The Evolutionary Tree, Transmission Of Tes, Transposition Mechanisms, Tes And Species Evolution