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Sequencing DNA - Overview, Maxam-gilbert Sequencing, Chain Termination Method, Automated Sequencing With Fluorescent Dyes

genetic gene sequence base protein

A gene is a segment of DNA that carries the information needed by the cell to construct a protein. Which protein that is, when it is made, and how damage to it can give rise to genetic disease all depend on the gene's sequence. In other words, they depend on how the building blocks of DNA, the nucleotides A, C, G, and T (adenine, cytosine, guanine, and thymine) are ordered along the DNA strand. For example, part of a gene may contain the base sequence TGGCAC, while part of another gene may contain the base sequence TCACGG. Knowing a gene's base sequence can lead to isolation of its protein product, show how individuals are related, or point the way to a cure for those people carrying it in its damaged form.

Severe Combined Immune Deficiency - Types And Severity Of Immunodeficiency Diseases, Scid, Gene Therapy [next] [back] Selection - Types Of Selection, Variation, The Importance Of The Environment, Artificial Selection, Selection In Humans

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Sequencing DNA - Overview, Maxam-gilbert Sequencing, Chain Termination Method, Automated Sequencing With Fluorescent Dyes

genetic gene sequence protein base depend contain
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A gene is a segment of DNA that carries the information needed by the cell to construct a protein. Which protein that is, when it is made, and how damage to it can give rise to genetic disease all depend on the gene's sequence. In other words, they depend on how the building blocks of DNA, the nucleotides A, C, G, and T (adenine, cytosine, guanine, and thymine) are ordered along the DNA strand. For example, part of a gene may contain the base sequence TGGCAC, while part of another gene may contain the base sequence TCACGG. Knowing a gene's base sequence can lead to isolation of its protein product, show how individuals are related, or point the way to a cure for those people carrying it in its damaged form.
Additional Topics
Sequencing DNA - Overview

In 1977 two methods for sequencing DNA were introduced. One method, referred to as Maxam-Gilbert sequencing, after the two scientists at Harvard University who developed the technique, uses different chemicals to break radioactively labeled DNA at specific base positions. The other approach, developed by Frederick Sanger in England and called the chain termination method (also called the Sanger me…
Sequencing DNA - Maxam-gilbert Sequencing

All DNA sequencing protocols must incorporate a method for making the DNA fragments generated in the reaction "visible"; they must be capable of being detected. For Maxam-Gilbert sequencing a technique called end labeling is used, in which a radioactive atom is added to the ends of the DNA fragment being sequenced. The first step in this process is to use an enzyme, called a restrict…
Sequencing DNA - Chain Termination Method

Because it employs fewer steps and does not require the use of restriction enzymes, the chain termination method of DNA sequencing is used in more laboratories than is the Maxam-Gilbert approach. Chain termination sequencing is a clever variation of the reaction used to replicate DNA, and requires only a handful of components. Four reaction tubes, designated "A," "C," &…
Sequencing DNA - Automated Sequencing With Fluorescent Dyes

As originally developed, both the Maxam-Gilbert and the chain termination methods of DNA sequencing require the use of radioisotopes to visualize the fragments generated in the reactions. However, in addition to being a health risk and presenting a disposal problem, the use of radioactivity makes automation of the DNA sequencing process difficult. Machines that could automatically read DNA sequenc…
Severe Combined Immune Deficiency - Types And Severity Of Immunodeficiency Diseases, Scid, Gene Therapy [next] [back] Selection - Types Of Selection, Variation, The Importance Of The Environment, Artificial Selection, Selection In Humans
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Vote down Vote up

over 6 years ago


Sequencing DNA - Overview, Maxam-gilbert Sequencing, Chain Termination Method, Automated Sequencing With Fluorescent Dyes

genetic gene sequence protein base depend contain
Ads by Google
Used Biotech Instruments
PCR/RT PCR, DNA seq(ABI3730,3100)
Storm860/Typhoon9400. Centrifuge
www.cgenetool.com
Need Next Gen Sequencing?
Certified Next Gen Seq Provider
that can Help Advance Your Research
www.ambrygen.com/NextGenSequencing
DNA Sequencing Software
Easy, Intuitive, and Integrated
Try A Fully Functional Demo!
www.clcbio.com
SelectaDNA Spray
A Spray of DNA to
Keep the Robbers Away
www.selectadna.co.uk

A gene is a segment of DNA that carries the information needed by the cell to construct a protein. Which protein that is, when it is made, and how damage to it can give rise to genetic disease all depend on the gene's sequence. In other words, they depend on how the building blocks of DNA, the nucleotides A, C, G, and T (adenine, cytosine, guanine, and thymine) are ordered along the DNA strand. For example, part of a gene may contain the base sequence TGGCAC, while part of another gene may contain the base sequence TCACGG. Knowing a gene's base sequence can lead to isolation of its protein product, show how individuals are related, or point the way to a cure for those people carrying it in its damaged form.
Additional Topics
Sequencing DNA - Overview

In 1977 two methods for sequencing DNA were introduced. One method, referred to as Maxam-Gilbert sequencing, after the two scientists at Harvard University who developed the technique, uses different chemicals to break radioactively labeled DNA at specific base positions. The other approach, developed by Frederick Sanger in England and called the chain termination method (also called the Sanger me…
Sequencing DNA - Maxam-gilbert Sequencing

All DNA sequencing protocols must incorporate a method for making the DNA fragments generated in the reaction "visible"; they must be capable of being detected. For Maxam-Gilbert sequencing a technique called end labeling is used, in which a radioactive atom is added to the ends of the DNA fragment being sequenced. The first step in this process is to use an enzyme, called a restrict…
Sequencing DNA - Chain Termination Method

Because it employs fewer steps and does not require the use of restriction enzymes, the chain termination method of DNA sequencing is used in more laboratories than is the Maxam-Gilbert approach. Chain termination sequencing is a clever variation of the reaction used to replicate DNA, and requires only a handful of components. Four reaction tubes, designated "A," "C," &…
Sequencing DNA - Automated Sequencing With Fluorescent Dyes

As originally developed, both the Maxam-Gilbert and the chain termination methods of DNA sequencing require the use of radioisotopes to visualize the fragments generated in the reactions. However, in addition to being a health risk and presenting a disposal problem, the use of radioactivity makes automation of the DNA sequencing process difficult. Machines that could automatically read DNA sequenc…
Severe Combined Immune Deficiency - Types And Severity Of Immunodeficiency Diseases, Scid, Gene Therapy [next] [back] Selection - Types Of Selection, Variation, The Importance Of The Environment, Artificial Selection, Selection In Humans
Citing this material

Please include a link to this page if you have found this material useful for research or writing a related article. Content on this website is from high-quality, licensed material originally published in print form. You can always be sure you're reading unbiased, factual, and accurate information.

Highlight the text below, right-click, and select “copy”. Paste the link into your website, email, or any other HTML document.