Other Free Encyclopedias » Medicine Encyclopedia » Genetics in Medicine - Part 1 » Chromosomal Banding - Chromosome Structure, Chromosome Banding Techniques, Other Banding, Fluorescence In Situ Hybridization

Chromosomal Banding - Other Banding

chromosome light dna bands staining

R-banding is the reverse pattern of G bands so that G-positive bands are light with R-banding methods, and vice versa. R-banding involves pretreating cells with a hot salt solution that denatures DNA that is rich in adenine and thymine. The chromosomes are then stained with Giemsa. R-banding is helpful for analyzing the structure of chromosome ends, since these areas usually stain light with G-banding.

C-banding stains areas of heterochromatin, which is tightly packed and repetitive DNA. NOR-staining, where NOR is an abbreviation for "nucleolar organizing region," refers to a silver staining method that identifies genes for ribosomal RNA that were active in a previous cell cycle.

Chromosomal Banding - Fluorescence In Situ Hybridization [next] [back] Chromosomal Banding - Chromosome Banding Techniques

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